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Original link:Metabolite - Animal Model of Metabolic Diseases
The Animal Experiment Department of Heyuan Biology can provide animal models of obesity, diabetes, cardiovascular diseases, inflammation and other metabolic and circulatory diseases in rats and mice.
1. Liver injury model
Liver disease is a general term for all diseases that occur in the liver. It includes infectious diseases, tumor diseases, vascular diseases, metabolic diseases, toxic diseases, autoimmune diseases, genetic diseases, intrahepatic biliary lithiasis, etc. Infectious diseases include viral infection, bacterial infection, parasitic infection, etc., such as viral hepatitis, liver echinococcosis, etc. Tumors are divided into benign tumors and malignant tumors, such as liver cancer, hepatic hemangioma, hepatic lipoma, hepatic sarcoma, etc. The application range of animal models for liver disease is very wide.
Carbon tetrachloride induced liver injury model: A mouse model was established by intraperitoneal injection of different doses of carbon tetrachloride (CCl4) to induce acute liver injury in mice, and changes in plasma transaminase levels were detected.
Cholestasis type mouse liver injury model: Inducing a cholestasis type mouse liver injury model through bile duct ligation.
2. Establishment of renal ischemia-reperfusion injury model
Chronic renal failure (CRF) model: Hemodynamic changes in residual nephrons after 5/6 nephrectomy in rats lead to hyperfiltration proteinuria in residual nephrons, resulting in CRF characterized by glomerulosclerosis.
Acute kidney injury (AKI) model: AKI is a common critical illness involving multiple disciplines in clinical practice, with a prevalence rate of 3% to 10% in general hospitals and 30% to 60% in intensive care units. The mortality rate of critically ill patients is as high as 30% to 80%, and about 50% of surviving patients have permanent kidney injury. The prevention and treatment situation is severe. Establishing a stable AKI animal model is crucial for early detection and prevention of kidney disease research.
3. Colitis model
There are many types of gastrointestinal diseases, including ulcerative colitis, chronic enteritis, gastric bleeding, and gastric perforation, among which ulcerative colitis is currently a research hotspot. Constructing a stable animal model of ulcerative colitis is beneficial for studying and elucidating the pathogenesis of ulcerative colitis with unclear etiology and developing therapeutic drugs.
DSS induced mouse ulcerative colitis model: Seven days after modeling, the mouse colon shortened, intestinal wall thickened, intestinal mucosal congestion, local bleeding, and obvious ulcerative surface were observed, with significant lesions in the cecum and distal colon. The HE staining results showed that the DSS group had mucosal loss, incomplete glandular tissue, extensive infiltration of inflammatory cells, and typical inflammatory changes. Weight changes: 2.5% DSS induced weight loss, decreased appetite, and poor activity in mice.
4. Arthritis model
Induction of arthritis rat and mouse models using complete Freund's adjuvant. Arthritis refers to an inflammatory disease that occurs in the joints and surrounding tissues of the human body, caused by inflammation, infection, degeneration, trauma, or other factors. It can be divided into dozens of types.
The animal model of compound drug-induced arthritis has the advantages of simple and feasible modeling method, minimal impact on animals, and easy and time-saving operation. After successful modeling, the animal joints showed significant swelling, which is similar to the symptoms of human arthritis. The modeling process is simple, stable, and the clinical, pathological, and immunological changes are similar to those of human arthritis, making it an ideal animal model for arthritis.
5. Cardiovascular disease model
Coronary artery ligation induced myocardial ischemia models in rats and mice, including efficacy testing services for acute myocardial infarction, chronic myocardial infarction, chronic heart failure, and myocardial ischemia-reperfusion, mainly including modeling, efficacy testing, efficacy evaluation, etc.
