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Original link:Metaorganisms - Tumor Animal Models
Tumor animal models play a crucial role in basic research on tumor occurrence, development, metastasis, and drug development and treatment. Therefore, tumor research cannot be separated from the establishment of animal models. There are several commonly used tumor models in scientific research, including subcutaneous tumor formation, in situ tumor formation, and tail vein injection model
Subcutaneous tumor model
A tumor animal model established by directly seeding tumor cells (or tumor tissue) subcutaneously in mice. This tumor model is a simple and important tool for preclinical evaluation of drug efficacy in vivo, and also plays an important role in the study of tumor pathogenesis and drug action.
Subcutaneous tumor formation
This model is easy to operate, allowing for intuitive observation of tumor growth and convenient detection of important data such as animal weight, tumor growth curve, and tumor weight;
In situ transplantation model
In situ transplantation refers to the transplantation of tumor cells or tumor tissue blocks into the tissues and organs of immunodeficient animals, resulting in the formation of tumors and spontaneous metastases.
The method is to directly inoculate diluted tumor cells into the submucosa of the organ. Alternatively, tumor cells can be subcutaneously inoculated to a volume of around 500-800mm3, and the tumor tissue can be removed and cut into small pieces of 1-3mm3. The prepared cancer tissue block can be implanted under the serosa of the organ, and the serosa can be sutured to form a heterologous in situ tumor.
Commonly used in situ vaccinations include: brain, liver, muscle, and breast pad in situ.
The in situ transplantation model can better simulate the microenvironment of tumor cell growth in vivo, and simulate the process of tumor growth and even metastasis.
Tumor metastasis model by tail vein injection
After injection into the tail vein, tumor cells first enter the arterial blood circulation system through the pulmonary capillary network, which can cause multiple metastases throughout the body. However, due to the viscosity and tendency of tumor cells to aggregate, they are generally trapped in the microvessels of mouse lungs, mainly forming lung metastases, which may later lead to metastasis to distant organs. Mainly used to establish tumor metastasis (blood pathway) models, blood cancer models, or tumor lung metastasis.
Tail vein injection of breast cancer cells
Tumor metastasis is the most common cause of tumor mortality in clinical practice, but the mechanism and process of tumor metastasis are very complex and currently not fully understood. Establishing a metastasis model can better simulate the occurrence and development process of tumors in humans, and has higher predictive power for drug screening and development.
The Heyuan Shanghai Animal Experiment Platform can provide various tumor animal model constructions, pathological sectioning, drug efficacy testing, small animal imaging, and other services such as subcutaneous tumor formation, in situ tumor formation, and tail vein injection metastasis tumor formation.
