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Original link：Metaorganisms - Autophagy
Autophagy is the process by which cells utilize lysosomal degradation pathways for self ingestion, and is the main metabolic pathway for cells to maintain survival under stress such as malnutrition. Autophagy is mainly divided into three categories, namely macroautophagy, microautophagy, and chaperone mediated autophagy. Autophagy plays an important role in various diseases such as tumors, neurodegenerative diseases, and cardiovascular diseases.
The process of autophagy is as follows:
Figure 1 Autophagy process
Introduction to Autophagy Products:
1. Autophagy screening system：GFP-LC3 WT/MT、GFP-LC3-RFP-LC3 MT、Luciferase LC3 WT/Luciferase LC3 MT
Figure 2 Autophagy screening system
2. Autophagy detection system:
Single fluorescence LC3 virus：pLenti-CMV-EGFP-LC3-PGK-Puro、pLenti-Ubc-EGFP-3FLAG-LC3、pLenti-EF1a-GFP-LC3-PGK-Puro
Double fluorescence LC3 virus: AdV-mCherry-GFP-LC3 (efficient infection), LV-mCherry GFP-LC3 (stable expression), AAV-mCherry GFP-LC3 (in vivo detection), Western Blot detection of LC3 I/II, p62: provides LC3, p62 (antibody detection)
Figure 3 Autophagy detection system
3. Overexpression of autophagy genes/interference with lentivirus:
Interference with lentivirus, 3 targets/genes, interference efficiency of over 70%
Overexpression of lentivirus, eukaryotic expression vector ORF cloning, corresponding detection antibodies
Figure 4 Autophagy gene manipulation
