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Original link:Metaorganisms - Gene Interference
RNA interference (RNAi) is a phenomenon in normal organisms that inhibits the expression of specific genes. When double stranded RNA (dsRNA) homologous to the coding region of endogenous mRNA is introduced into cells, the mRNA undergoes degradation, leading to gene expression silencing. The antisense strand of small interfering RNA (siRNA) produced by exogenous dsRNA entering cells forms an RNA induced silencing complex (RISC) with various nucleases. RISC mediates RNA interference by binding and cleaving mRNA.
Carrier selection (partial)
The selection of viral vectors should refer to lentiviruses, adenoviruses, adenoviruses, retroviruses, etc. according to experimental needs.
Carrier category | number | Carrier element |
Slow virus interference | GL401 | pCLenti-U6-shRNA-CMV-Puro-WPRE |
GL404 | pCLenti-U6-shRNA-CMV-EGFP-WPRE | |
GL407 | pCLenti-U6-shRNA-CMV-EGFP-F2A-Puro-WPRE | |
GL408 | GL408 pCLenti-U6-shRNA-CMV-mCherry-F2A-Puro-WPRE | |
H7615 | pCLenti-U6-shRNA-CMV-mCherry-F2A-BSR-WPRE | |
Adenovirus interference | DKD001 | pADV-U6-shRNA-CMV-EGFP |
DKD004 | pADV-U6-shRNA-CMV-mCherry | |
Adenovirus interference | AKD001 | pAAV-CBG-EGFP-3xFLAG-WPRE-H1-shRNA |
AKD003 | pAAV-CBG-mCherry-3xFLAG-WPRE-H1-shRNA | |
H12663 | pAAV-U6-shRNA/spgRNA v2.0-CMV-EGFP-WPRE |
Service Introduction
Based on the target gene provided by the customer, design three siRNA targets for this gene, and then construct these targets into relevant vectors. For virus vectors, the packaged virus particles can be directly used for infecting cells or animal experiments.
Virus Carrier Service Process
