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Original link:Genomics - Knockout Cell Line Construction
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 is an adaptive immune defense mechanism formed by the long-term evolution of bacteria and archaea. The CRISPR/Cas9 system can be used for editing various genomes. The CRISPR/Cas9 system recognizes the cleavage region through small guide RNA (sgRNA) and uses Cas9 endonuclease to perform predicted cleavage in the middle of the recognition site, causing DNA double strand breaks and random mutations after cell error repair. The CRISPR/Cas9 system has high mutation efficiency, flexibility, simplicity, short cycle, and low cost, and has been widely used in gene editing research. The application areas of CRISPR/Cas9 system are gene knockout strain construction and gene knockout animal research.
The company can provide project services including: CRISPR/Cas9 experimental protocol design, CRISPR/Cas9 gene knockout vector construction, CRISPR/Cas9 gene knockout vector virus packaging, CRISPR/Cas9 gene knockout cell line construction
Service features:
1. To customize a specific gene knockout cell line, only the gene name needs to be provided;
2. Quickly obtain effective sgRNA with high gene editing efficiency;
3. Short experimental period;
4. CRISPR/Cas9 lentivirus packaging services can be provided as needed
Operation process
Design, vector construction, and activity verification of sgRNA targeting the target sequence
2Co transfection of target cell lines and preliminary screening of mixed clones
3 Preparation of monoclonal antibodies, screening and identification of cell genotypes using monoclonal antibodies
4 Amplification of positive cell lines
