Pre stained protein marker: a convenient and efficient tool for protein separation and identification
Pre stained protein marker, also known as pre fabricated protein molecular weight marker, is a biochemical reagent used for protein electrophoresis separation and protein molecular weight determination. It provides a convenient and efficient protein separation and identification tool for experimenters by pre staining proteins with known molecular weights to form a series of standard bands with different molecular weights.
1、 The working principle of pre stained protein marker
The proteins in the pre stained protein marker have been purified and identified, and then stained with specific staining agents. After binding with proteins, these dyes can exhibit different colors or fluorescence during protein electrophoresis. During the electrophoresis separation process, the pre stained protein marker is electrophoresed together with the test sample. Based on the migration rates of each band in the marker, the molecular weight of the test protein can be estimated.
2、 Application areas of pre stained protein markers
1. Protein electrophoresis: Pre stained protein Marker is widely used in protein electrophoresis experiments, such as SDS-PAGE (polyacrylamide gel electrophoresis) and Western blot. By comparing the migration rates of the standard bands and the protein bands to be tested in the Marker, the molecular weight of the protein can be quickly and accurately identified.
2. Protein purification: During the protein purification process, pre stained protein markers can be used to monitor the molecular weight and purity of proteins in the purification steps, ensuring the smooth progress of the purification process.
3. Protein expression: Pre stained protein markers can be used to detect protein expression levels. By comparing the intensity of target protein bands in different samples, the expression level of proteins can be evaluated.
4. Gene cloning and mutation: Pre stained protein markers can also be used to verify the success of gene cloning and mutation. By comparing the molecular weight of wild-type and mutant proteins, it is possible to determine whether a mutation has occurred.
3、 The advantages of pre stained protein markers
1. It eliminates the tedious staining and decolorization steps in traditional protein electrophoresis, greatly simplifying the experimental operation process.
2. The standard bands are clearly visible, easy to observe and compare, and improve the accuracy of protein molecular weight estimation.
3. Suitable for various protein electrophoresis systems, such as SDS-PAGE, Western blot, etc., with high universality.
4. The use of environmentally friendly dyes reduces the harm to laboratory personnel and the environment.
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