Li Ji Biological Pre stained Protein Molecular Weight Standards (Classification and Selection of Protein Markers)
Protein marker, also known as protein molecular weight standard, is a mixture of several proteins with known molecular weights, used like a "ruler" to indicate the size of protein bands. In the Western Blot experiment, the protein marker is mainly used to indicate the molecular weight of the protein band. In addition, the protein marker can also be used to help judge whether the protein is successfully transferred to the membrane or the electrophoresis progress of the protein on the gel. Therefore, selecting a suitable protein marker is also one of the important conditions for the success of the experiment.
Li Ji Biological Pre stained Protein Molecular Weight Standard
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Pre stained proteins have different apparent molecular weights in different buffer systems, and the above two images are electrophoresis diagrams under two different buffer systems. Suitable as a protein molecular weight standard for SDS-PAGE and Western Blot. Can be used as a reference for protein molecular weight size in SDS-PAGE and Western Blot experiments.
Classification of protein markers
Unpainted Marker: Unpainted protein markers cannot be seen during electrophoresis, and can only serve as indicators after protein staining after electrophoresis is completed. They cannot provide predictive information during the experimental process. However, since the protein does not have attached dye molecules or labeling molecules, the size shown is exactly the size of the protein itself, making it the most accurate.
Pre dye marker: containing dye and protein, visible to the naked eye, can directly observe the electrophoresis process and whether the membrane transfer is complete, and is commonly used. However, the covalent coupling between Marker protein and dye results in a change in the migration efficiency of protein molecules in electrophoresis, and the molecular weight indicated by it is not an exact value with some error.
Exposure Marker: In the exposure step, both the target strip and the marker strip can be exposed and displayed simultaneously, reducing errors and overcoming changes in pre dyed marker mobility, but at a high cost.
Selection of protein markers
• Scope coverage
When selecting a protein marker, ensure that its molecular weight range covers the size of the target protein being studied. Generally speaking, selecting standards with smaller, medium, and larger molecular weights to cover proteins of different sizes.
Double marker
Some markers are dual labeled and can be stained during electrophoresis, making them suitable for dual color labeling applications such as fluorescence and chemiluminescence, providing more experimental options.
• Quality and stability
Choose high-quality and stable protein markers from reliable suppliers to ensure consistency and accuracy in their performance.
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