The difference between tissue cryopreservation solution and cell cryopreservation solution, which one is more effective?
Cell cryopreservation technology refers to the preservation of live cells at low temperatures to maintain their vitality, so that they can be retrieved for recovery when needed. Cell cryopreservation is one of the main methods for cell preservation, and the application range of cell cryopreservation solution is very wide. In the field of scientific research, it is widely used in cell preservation, cell culture, and cell passage. Researchers can use it to preserve different types of cells for future experiments. Below, we will briefly introduce two commonly used cryopreservation solutions and see the difference between tissue cryopreservation solution and cell cryopreservation solution.
Introduction to Organizational Cryopreservation Liquid and Cell Cryopreservation Liquid Products
1. Tissue cryopreservation solution: widely used for the transportation of high fidelity samples such as primary tissues, transplanted organs, and single-cell sequencing. Traditional methods of sample preservation and transportation, such as ultra-low temperature storage and chemical fixation, often result in the degradation of nucleic acid in tissue cells, reduced transcriptome integrity, cell rupture, and contamination with free RNA. Using the product to completely immerse the sample and infiltrate into the tissue, transporting the sample at 4 ℃ ensures the integrity of precious sample information and reduces batch differences caused by transportation; Simultaneously providing an ideal microenvironment for cells and tissues to avoid improper sample preservation leading to cell apoptosis and tissue necrosis; After storing cells or tissues at 4 ℃ for 72 hours, the cell activity can be maintained at over 90% and the tissue can remain intact.
2. Cell cryopreservation solution: Suitable for the vast majority of tumor cells and conventional cells, most stem cells, and cryopreserved cells can be stored at -80 ℃ for a long time. Free from animal derived proteins and serum components, it can effectively reduce contamination from various bacteria, viruses, and mycoplasma, ensuring the safety of frozen cells. This cryopreservation solution contains various cellular nutrients such as DMSO and glucose, which improve cell survival rate and vitality. It is also suitable for serum-free culture of cells and protein expressing cells.
Preservation effect of tissue cryopreservation solution and cell cryopreservation solution products
Cell cryopreservation solution: emphasizes the high resurrection rate and vitality of cells, ensuring that cell function is not affected.
Organizational cryopreservation reagent: focuses on long-term preservation of the structural integrity and functional activity of tissues, with cell viability maintained at over 90% after recovery.
Storage conditions for tissue cryopreservation solution and cell cryopreservation solution when not in use
Cell cryopreservation solution: transported at room temperature. Stored at 4 ℃, with a shelf life of 24 months.
Organizational freezing solution: blue ice transportation- Store at 20 ℃ away from light, with a shelf life of 12 months. It can be stored at 4 ℃ for two weeks, and the color will change from colorless to light brown within two weeks of storage at this temperature, without affecting the use of the product.
Precautions for the use of tissue cryopreservation solution and cell cryopreservation solution products
▶ Organizational freezing solution:
1. The product should be thawed at 4 ℃ before use. After melting, there will be a small amount of flocculent material. Shake until the flocculent material is completely dissolved before use.
2. Suggest packaging the product according to actual usage to avoid repeated freezing and thawing.
3. During storage, it should be sealed and used with caution if the inner packaging is found to be damaged.
▶ Cell cryopreservation solution:
1. After cells are added to the frozen storage solution and packaged, the storage time outside should be reduced, and they should be transferred to a -80 ℃ ultra-low temperature freezer as soon as possible.
2. For cryopreservation of stem cells (ES cells), it is recommended to perform experimental cell cryopreservation culture on the frozen cells for at least one week before use, confirm their performance, and then proceed with formal cryopreservation.
3. The product will contain 10% DMSO. For cells that are sensitive to DMSO, it is recommended to conduct at least one week of experimental cell cryopreservation culture of this product to confirm its performance before formal cryopreservation.
4. When cells are revived, the frozen cells taken out should be immediately placed in a 37 ℃ water bath and gently shaken. Rapid thawing can help improve recovery efficiency.
5. For fragile or precious cells, it is recommended to store them overnight at -80 ° C and then transfer them to liquid nitrogen tanks for long-term storage.
In summary, both cell cryopreservation solution and tissue cryopreservation solution are important biomaterials that play an irreplaceable role in scientific research and medical fields.
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