Lipid peroxidation sensor as a 'fluorescent sentinel' for monitoring cellular health
Lipid peroxidation is the process of oxidative degradation of lipid molecules in organisms under the action of reactive oxygen species (ROS), which is closely related to biological phenomena such as cell aging, disease occurrence, and cell death. In order to accurately monitor the level of lipid peroxidation within cells, scientists have developed an efficient tool - a lipid peroxidation sensor.
Lipid peroxidation sensors are typically constructed based on a fluorescent dye called C11-BODIPY 581/591. This dye has good photostability and low fluorescence artifact characteristics, and can accumulate in the cell membrane, making it an ideal choice for monitoring lipid peroxidation processes. When intracellular lipids undergo peroxidation, the polyunsaturated dienyl portion of C11-BODIPY 581/591 is oxidized, resulting in a significant change in fluorescence emission wavelength. Specifically, in the reduced state, the excitation and emission wavelengths of the dye are 581nm and 591nm, respectively, exhibiting red fluorescence; After oxidation, its excitation and emission wavelengths shift to 488nm and 510nm, and the fluorescence color changes to green. The change in fluorescence color can intuitively reflect the degree of lipid peroxidation within cells.
Lipid peroxidation sensors have broad application value in biomedical research. Through techniques such as fluorescence microscopy or flow cytometry analysis, scientists can monitor lipid peroxidation levels in live cells in real-time, and study their relationship with biological processes such as cell aging and disease occurrence. In addition, the sensor can also be used to evaluate the effectiveness of antioxidants, providing strong support for the development of new antioxidant drugs.
When using a lipid peroxidation sensor, the following points should be noted: the sensor should be dissolved in high-quality dimethyl sulfoxide (DMSO) and stored in the dark at low temperatures; During use, avoid contact with skin, eyes, and mucous membranes, and wear gloves for operation; Select appropriate concentration and staining time according to experimental requirements to ensure the accuracy of experimental results.
In summary, lipid peroxidation sensors play an important role in biomedical research as an efficient and sensitive monitoring tool. Not only can it reveal the dynamic changes of intracellular lipid peroxidation, but it also provides a new perspective and method for studying related biological phenomena and disease mechanisms. With the continuous development of technology, lipid peroxidation sensors are expected to be widely applied in more fields and make greater contributions to human health.
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