At present, more and more biological products are produced using animal cell culture technology, further expanding the application scope of cell culture technology. Fetal bovine serum (FBS), as a key component of animal cell culture medium, plays an important role in animal cell culture. The functions of fetal bovine serum are roughly as follows:
The role of fetal bovine serum
1： Provide nutrients to cells
In today's cell culture, there are very few nutrients in the basic culture medium, so it is necessary to use characteristic fetal bovine serum to provide growth maintaining hormones in order to maintain better cell growth. In addition, professional fetal bovine serum can provide binding proteins that can effectively identify vitamins and fats, thereby providing targeted nutrients to ensure the activity of cell growth.
2： Protect cells from damage
During the process of cell culture, special attention should be paid to protecting the cells. Therefore, the use of professional fetal bovine serum can effectively provide protease inhibitors, which can effectively treat the remaining protease during cell division, ensuring that the cells that need to be cultured and grown are not damaged.
3： Plays a detoxifying role
In cell culture, there may be some toxic metals and pyrogens that can cause toxicity to cells, and fetal bovine serum contains abundant binding proteins that can bind to these substances and release toxic substances to achieve detoxification. This ensures that better results can be maintained in the use of fetal bovine serum, maximizing the effectiveness of fetal bovine serum in cultivating cells.
What are the uses of fetal bovine serum
Bovine serum is a natural medium that is widely used in cell culture and contains abundant nutrients necessary for cell growth. It is commonly used in the in vitro culture of animal cells and plays an important role in providing hormones that maintain cell exponential growth, nutrients that are not present or present in basic culture media, and major low molecular weight nutrients.
It can provide binding proteins and recognize vitamins, lipids, metals, and other hormones, and can bind or modulate the activity of the substances they bind to. In some cases, binding proteins can bind with toxic metals and thermogens to exert detoxification effects.
Bovine serum is the source of factors required for cell adhesion and spreading on plastic culture media. Bovine serum provides hormones for maintaining cell exponential growth, nutrients that are not present or present in minimal amounts in the basal culture medium, and primarily low molecular weight nutrients. Cow serum can act as an acid-base buffer. Provide protease inhibitors to protect cells from damage when residual trypsin is inactivated during cell passage.
Introduction and purchase of fetal bovine serum products
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