During the process of cell culture, contamination by black gum mites has always been a headache. These tiny pollutants appear as small black dots under the microscope, sometimes in the form of dots, sometimes in small flakes, vibrating in place in a Brownian motion like manner, which has a serious impact on cell growth and experimental results. In order to effectively address this challenge, a black fungus removal kit has been developed, providing strong support for cell culture work.
1、 Composition and principle of black glue insect removal kit
The black gum worm removal kit usually contains one or more highly effective cleaning reagents for black gum worms. The main components of these reagents are antibiotics or other compounds with bactericidal effects, which can specifically act on black glue worms without affecting the normal growth of cells. In addition, the reagent kit may also contain some auxiliary reagents, such as PBS buffer, complete culture medium, etc., for cell cleaning and reagent dilution.
The working principle of the reagent kit is to effectively control and kill black gum mites in cells, serum, and culture medium by eliminating the bactericidal effect of the reagent, thereby restoring the normal growth environment of cells. At the same time, some reagents also have a certain clearing effect on common bacteria, further improving the success rate of cell culture.
2、 Instructions for using the black glue insect removal kit
When using the black glue insect removal kit, certain operating steps need to be followed. Firstly, ensure that the cell culture environment is clean and avoid cross contamination. Prepare the necessary reagents and equipment, such as pipettes, centrifuge tubes, microscopes, etc. According to the instructions of the reagent kit, dilute the reagent to the appropriate concentration and discard the old culture medium. Wash the cells thoroughly with PBS buffer. Add the diluted clearing reagent to a new culture medium, and then add it to the cell culture dish to ensure uniform distribution of the reagent and avoid mechanical damage to the cells. Place the processed cells in a culture incubator and regularly observe the cell status and clearance effect. According to the instructions, it may be necessary to handle multiple cycles continuously.
3、 Precautions for the Black Gum Insect Removal Kit
When using the black glue insect removal kit, the following points should be noted:
Strictly adhere to sterile operating procedures to avoid introducing new sources of contamination.
The dilution ratio of reagents varies depending on the reagent brand and cell type, and it is necessary to carefully read the instructions and strictly follow the guidance for operation.
Cell toxicity testing should be conducted before processing. Although the black gum worm removal reagent is non-toxic or low toxic to the cells themselves, attention should still be paid to its impact on cell growth.
The treatment cycle may vary depending on the degree of pollution and cell type, and needs to be adjusted according to the actual situation.
Reagents usually need to be stored at specific temperature conditions (such as -20 ℃) and avoid repeated freeze-thaw cycles to ensure their activity.
4、 Summary and Prospect
The black glue insect removal kit, as a clean guard in cell culture, provides an effective solution for researchers. With the continuous advancement of technology and the increasing demand for cell culture quality, the performance and effectiveness of the black fungus removal kit will also continue to improve.
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