Cell transfection total rollover? High efficiency low toxicity transfection reagent free!
Cell transfectionLiposome transfection is one of the most commonly used transfection methods for studying the gene function of eukaryotic cells by introducing exogenous molecules into cells. However, the role of transfection reagents is far underestimated.
Have you ever experienced the classic scenario of experimental failure caused by inadequate transfection reagents?
Scenario 1
After transfecting cells with fluorescent plasmids, there was still only weak fluorescence after 48 hours.
Possible reasons: Weak fluorescence is generally judged as low transfection efficiency, which may be due to poor quality of transfection reagents, substandard quality and concentration of transfected plasmids, low cell density or poor cell state during transfection.
Solution: When choosing a good transfection reagent, it is best to choose a validated commercial transfection reagent; Under normal circumstances, the concentration of plasmids used for cell transfection should be greater than 500 ng/ul; The timing of cell transfection should be during the logarithmic growth phase of the cell, and the optimal cell density is generally around 90% to 95%.
Scenario 2
After 24 hours of observation after cell transfection, it was found that the cells had begun to die.
Possible reasons: Cell death after transfection may be due to the high toxicity of the transfection reagent, or the addition of antibiotics during transfection, or the failure to change the transfection solution properly.
Solution: Choose a transfection reagent with less cytotoxicity to cells, replace the culture medium containing antibiotics before transfection, and change the medium 4-6 hours after transfection.
To do a good job, one must first sharpen their tools! A good transfection reagent can save us a lot of obstacles in our experimental journey!
Li Ji Biotechnology supports scientific research, and from now until May 31, 2024, the transfection reagents will be tested for free!
▼EZ Trans Cell Transfection Reagent (Efficient)
As a new generation of transfection reagents, its transfection principle is that positively charged cationic polymers and negatively charged phosphate groups in nucleic acids form a positively charged complex, which interacts with negatively charged proteoglycans on the cell surface and enters the cell through endocytosis.
It has the characteristics of high transfection efficiency, low cytotoxicity, simple operation, good reproducibility, and wide applicability.
▼EZ Trans cell transfection reagent (low toxicity)
As a new generation of transfection reagents, its transfection principle is that positively charged cationic polymers and negatively charged phosphate groups in nucleic acids form a positively charged complex, which interacts with negatively charged proteoglycans on the cell surface and enters the cell through endocytosis.
It has the characteristics of high transfection efficiency, low cytotoxicity, simple operation, good reproducibility, and wide applicability.
Compared to products such as Thermo/Lipo 3000/Invotrigen/Lipofectamine 3000, the operation is more convenient
▼EZ Trans Plus Cell Transfection Reagent (II)
As a new generation of transfection reagents, its transfection principle is that positively charged polymers and negatively charged phosphate groups of nucleic acid molecules form a positively charged complex, which interacts with negatively charged proteoglycans on the cell surface and enters the cell through endocytosis.
It has the characteristics of high transfection efficiency, low cytotoxicity, simple operation, good reproducibility, and wide applicability.
▼EZ Trans Lipo Cell Transfection Reagent (Liposome)
EZ Trans Lipo encapsulates nucleic acids in nanoliposomes through a special delivery mechanism, transferring exogenous DNA, RNA, and siRNA into various types of cells. It can transfect adherent cells and suspension cells, and can also be used for gene knockout experiments and gene expression studies of siRNA and shRNA.
The advantages of high transfection efficiency, low cytotoxicity, wide applicability to cells, and high cost-effectiveness.
▼EZ Trans RNA transfection reagent
The use of novel liposome nanomaterials can be used to transfect small molecule RNA and DNA within 150bp, such as siRNA and miRNA. The transfected cells include the vast majority of adherent cells, such as general cell lines and tumor cell lines.
This transfection reagent is a fast, efficient, and ready to use reagent solution. Simply mix the cell culture medium with siRNA or miRNA transfection target small RNA and transfection reagent in an EP tube, incubate at room temperature for about 2 minutes, and then add it to the complete cell culture medium. The serum has no effect on the transfection effect, and the experimental process does not require deliberate addition or replacement of the culture medium.
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