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What is the difference in inoculation quantity between suspended cells and adherent cells?
Most suspended cells are more difficult to color and have lower O.D. values compared to adherent cells. Therefore, it is generally necessary to increase the number of cells inoculated or extend the culture time after adding CCK-8. The color development of adherent cells is relatively easy. If the number of cells is too large, the absorbance may sometimes exceed the reading of the enzyme-linked immunosorbent assay (ELISA) reader.