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What is the difference between BrdU method and CCK-8 method?

Pubtime：2024-05-10

View volume：193

The CCK-8 method reflects cell activity by detecting dehydrogenases in logarithmic growth stages, while the BrdU method detects DNA synthesis in cells. The CCK-8 method only requires the addition of reagents and can be detected by colorimetric method, while the BrdU method not only requires the addition of reagents but also the fixation of cells, which can be detected by luminescence method after antigen antibody reaction. The principles of the two are completely different. If only detecting cell proliferation, then the CCK-8 method is relatively simple.

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