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What supplements can be added to RPMI1640 culture medium?

Pubtime：2024-05-10

View volume：192

We usually add some supplements to RPMI1640 medium, such as growth factors, proteins, 10% fetal bovine serum, phenol red, bispecific antibodies, etc. These supplements are adjusted appropriately according to the type of cells being cultured. For example, if we want to observe the pH value of RPMI1640 medium more intuitively, we can add phenol red. Phenol red produces different colors according to changes in pH value, which is beneficial for researchers to adjust pH value in a timely manner. This is also one of the reasons why RPMI1640 medium changes color; When we want to prevent cell contamination, we can add a dual antibody solution appropriately for antibacterial purposes; We need abundant growth factors and nutrients, so we can simply add 10% fetal bovine serum to ensure that the nutrients in RPMI1640 medium are appropriate. The addition of supplements to RPMI1640 medium is based on our experimental design and objectives.

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