He Yuan Li Ji

The difference between PBS solution and DPBS solution

Pubtime：2024-05-10

View volume：94

PBS can provide a relatively stable ion environment and pH buffering capacity, and is generally used as a solvent to dissolve and protect reagents. It is a commonly used buffer salt solution in biology, used for molecular cloning, etc. DPBS is a balanced salt solution used to maintain cell activity in the short term. Maintain the structural and physiological integrity of ex vivo cells within a limited time. The pH is 7.2-7.4, which is similar to the pH of the human body. The solution has an osmotic pressure equivalent to that of human blood and does not contain Ca2+or Mg2+. It is suitable for various cell culture applications, such as washing cells before dissociation, transporting cells or tissues, diluting cells for counting, and preparing reagents. In fact, PBS is a general term for a type of phosphate buffered saline solution, although there are commonly used formulas, it is not limited to any one. DPBS is a PBS solution that Renato Dulbecco improved based on his own experimental needs. Due to its frequent use, it has been adopted as a relatively fixed name. Essentially, both belong to phosphate buffer solutions. According to commonly used formulas, the components of the two solutions are basically the same, with slightly different contents. It can be said that DPBS is a type of PBS.

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