He Yuan Li Ji

What should be noted for efficient cell cryopreservation?

Pubtime：2024-05-11

View volume：53

1. Slow freezing: Slow freezing can gradually dehydrate cells, and cells will not be damaged by the production of large ice crystals. On the contrary, the appearance of large crystals inside cells can cause damage and rupture of cell membranes and organelles. 2. Cell viability and concentration: Cells should be frozen in a state of good growth, density of about 80-90%, and viability of over 90%. Cells with poor cell viability have a low survival rate after cryopreservation, therefore, they must be cryopreserved during the period of vigorous cell division. 3. Freezing density: When the cell density is less than 5x105 live cells/mL during freezing, it is difficult to successfully recover. 4. Cryovial cap: The cap of the cryovial must be tightened, otherwise water will seep in and cause contamination during water bath recovery. 5. Freezing time: Cells cannot be stored in a -80 ℃ freezer for a long time. We suggest that the storage time in a -80 ℃ refrigerator should not exceed 48 hours.

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