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CCK-8 test solution toxic to cells?

Pubtime：2024-06-07

View volume：7

The CCK-8 solution itself is slightly toxic due to the presence of high concentrations of PMS. However, CCK-8 added to the medium was not toxic because it was diluted 10-fold. Therefore, prolonged incubation, such as overnight or for several days, is acceptable. The same cell culture medium after CCK -8 detection can also be used for other cell proliferation detection, such as crystal violet detection, neutral red detection or DNA fluorescence detection. Since each cell has a different tolerance to CCK-8, the viability of the cells after addition of CCK-8 in culture is first examined when a long period of culture is required.

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