Can primary cells be cryopreserved? What is the reason for the poor survival rate after cryopreservation and resuscitation?-Frequently asked questions-He Yuan Li Ji

Can primary cells be cryopreserved? What is the reason for the poor survival rate after cryopreservation and resuscitation?

Pubtime：2024-07-02

View volume：137

This depends on the type of cell. Some cell types, such as neurons, glial cells, and some slow growing epithelial cells, are not recommended for expansion culture and re freezing. Other cell types such as fibroblasts, astrocytes, renal mesangial cells, astrocytes, etc. can be expanded, cultured, and cryopreserved. However, it should be noted that the process of re freezing may lead to changes in cell growth performance. The low recovery rate after cryopreservation requires consideration of pre cryopreservation cell activity, cell volume, and cryopreservation system. The cryopreservation system refers to whether serum containing cryopreservation solution or one-step cryopreservation solution is used, and different cryopreservation methods correspond to different operating methods that need to be noted.

Previous：What is the reason why primary cells that adhere to the wall are difficult to digest?

Next：Which generation is generally better for conducting experiments on primary cells?