What are the advantages and disadvantages of BCA protein quantification method?-FAQ-He Yuan Li Ji

What are the advantages and disadvantages of BCA protein quantification method?

Pubtime：2024-12-19

View volume：5

advantage:

1. This method is widely used, easy to operate, fast, and can complete the test within 45 minutes. 4 times faster and even better than the classic Lowery method;

2. Not easily affected by general concentrations of detergents, urea, etc., with strong anti-interference ability;

3. The coefficient of variation for detecting different protein molecules is also much lower than that of the Coomassie Brilliant Blue method;

4. Accurate, high sensitivity, and good reagent stability. The BCA method has a good linear relationship in the concentration range of 20-20 ug/mL, and the range of trace BCA determination is 0.5-10 ug/ml.

5. Economical and practical, in addition to test tubes, measurements can be performed in microplate wells, greatly saving sample and reagent usage;

Disadvantages:

1. May be affected by chelating agents (EDTA, EGTA) and high concentrations of reducing agents (DTT, β - mercaptoethanol);

2. Incubate the reaction in a constant temperature water bath for 30 minutes or longer.

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