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Annexin VPI法如何避免出现假阳性结果?

Pubtime:2025-02-05
View volume:56

细胞接种不易过密:接种对数生长期的细胞时密度不要>1*106,以免细胞培养过程中自身引起凋亡,而非外界处理因素;避免消化过度:因消化过度可能会造成PS外翻,得到假阳性的结果;操作尽量轻柔:收集细胞时力度过大很可能造成细胞膜损伤,导致细胞膜的磷脂层暴露,从而结合Annexin V,导致假阳性,因此处理贴壁细胞时要小心操作,尽量避免人为的损伤。

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