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What are the steps for cell cryopreservation?

Pubtime：2024-04-02

View volume：153

Freezing method one: Place the freezing tube at 4 ° C for 30-60 minutes → (-20 ° C for 30 minutes *) → -80 ° C for 16-18 hours (or overnight) → Long term storage in liquid nitrogen tank vapor phase. Freezing method 2: Place the freezing tube in a programmable cooling machine that has been programmed to cool down 1-3 ° C to below -80 ° C per minute, and then store it in a liquid nitrogen tank vapor phase for long-term storage*- At 20 ° C, it should not exceed 1 hour to prevent excessive ice crystals from causing massive cell death. This step can also be skipped and placed directly in a -80 ° C freezer, but the survival rate will decrease.

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