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What concentration of CO2 should be used when cultivating cells? 5% or 10%, or no impact at all?
Most culture media use HCO3-/CO32-/H+as a pH buffer system, and the content of NaHCO3 in the medium will determine the CO2 concentration that should be used during cell culture. When the NaHCO3 content in the culture medium is 3.7g per liter, 10% CO2 should be used for cell culture; When NaHCO3 is 1.5g per liter in the culture medium, cells should be cultured with 5% CO2.