He Yuan Li Ji

What are the possible precipitates in serum?

Pubtime：2024-04-23

View volume：60

Based on years of experimental research, fetal bovine serum and other serum used for cell culture may contain the following types of precipitates: (1) fibrin, which is a frequently occurring large precipitate that can reach 1-2mm and can be observed with the naked eye. Because serum is collected and rapidly processed at low temperatures, some fibrinogen (soluble precursors that form flocculent fibrin) remains in a dissolved state during the processing. After the final filtration and packaging, fibrin precipitates will coagulate in the bottle. (2) Calcium phosphate, which is also a common precipitate, usually causes serum turbidity and increases when cultured at 37 ℃. This sediment appears as small black dots under an inverted microscope, which appear to be active due to Brownian motion and are often mistaken for microbial contamination. (3) Cholesterol, fatty acid esters, and some proteins. They are also a common cause of sediment in serum.

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