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Why are many cells difficult to adhere to the wall after revival?

Pubtime:2024-04-23
View volume:236

It may be due to poor state during cell cryopreservation or slow movement during recovery, leading to cell death. Remember to melt quickly and centrifuge to remove DMSO. If the non adherent cells still survive, they can be collected by centrifugation and re inoculated back into the original bottle, and the serum ratio can be adjusted to 15% to assist in adherent culture.

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