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After cell revival, only a very small number of cells adhere to the wall. Do we need to revive them?

Pubtime：2024-04-28

View volume：220

Don't rush to throw it away yet! Serum and cytokines can be added, or fresh growth medium can be changed every 2-3 days. After 2-3 medium changes, most cells can show significant proliferation. At this time, normal passage operations can be followed. Of course, if the cell inventory is sufficient or there is an urgent need to conduct experiments, a new cell line can be revived~

Previous：I have prepared too much cryoprotectant and don't want to waste it. Can I keep it for next use?

Next：Why was it that when I revived the cells, they were already attached to the wall, but when I went to see them the next day, I found that all the cells were dead?

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