New Product Launch | EZ Trans siRNA&mRNA Transfection Reagent Helps Scientific Research!
The transfection reagent series of He Yuan Li Ji has added a new force!!!
The newly launched products this time include:
① AC04L052-EZ Trans siRNA transfection reagent
② AC04L062-EZ Trans mRNA Transfection Reagent
There are all sizes and specifications available! Please refer to the following text for details:
New product one
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EZ Trans siRNA转染试剂
Item number: AC04L051 Specification: 300uL
Item number: AC04L052 Specification: 1mL
Introduction: This product is a novel transfection reagent developed based on cationic polymer, suitable for transfection of siRNA and microRNA (miRNA). It can achieve high efficiency and low toxicity transfection in various common cells, and has the advantages of easy operation and good repeatability, making it suitable for siRNA or miRNA mediated gene suppression experiments.
SiRNA gene knockdown effect display image
On the day before transfection, 293T and HT-29 cells stably expressing EGFP protein were seeded into 24 well plates. Perform siRNA transfection that interferes with EGFP protein expression according to the instructions of the siRNA transfection reagent. After 48 hours of transfection, the knockdown efficiency was observed and evaluated by fluorescence microscopy. The results showed that the transfected cells were in good condition, and the green fluorescence intensity was significantly reduced, indicating that the expression level of EGFP protein was successfully downregulated.
SiRNA WB plot
On the day before transfection, 293T cells stably expressing EGFP protein were seeded into 24 well plates. Perform siRNA transfection that interferes with EGFP protein expression according to the instructions of the siRNA transfection reagent. After 48 hours of transfection, the knockdown efficiency was evaluated by Western Blot. The results showed that the expression level of EFGP protein was significantly reduced.
SiRNA transfection efficiency display chart
On the day before transfection, 293T and Huh-7 cells were seeded separately into 24 well plates. Perform FAM labeled negative control siRNA transfection according to the operating instructions of the siRNA transfection reagent. After 4-6 hours of transfection, the transfection efficiency of each cell line was observed under a fluorescence microscope. The results showed that the transfected cells were in good condition and the fluorescence efficiency exceeded 90%.
On the day before transfection, 293T cells were seeded into a 24 well plate. According to the instructions of the siRNA transfection reagent, perform negative control siRNA transfection labeled with Cy3. After 4-6 hours of transfection, evaluate the transfection efficiency using fluorescence microscopy. The results showed that the transfected cells were in good condition and the fluorescence efficiency exceeded 90%.
MiRNA transfection efficiency display chart
On the day before transfection, 293T and Huh-7 cells were seeded separately into 24 well plates. Perform FAM labeled negative control microRNA mimetics single stranded transfection according to the operating instructions of siRNA transfection reagent. After 4-6 hours of transfection, the transfection efficiency of each cell line was observed under a fluorescence microscope. The results showed that the transfected cells were in good condition and the fluorescence efficiency exceeded 90%.
New product two
EZ Trans mRNA转染试剂
Item number: AC04L061 Specification: 300uL
Item number: AC04L062 Specification: 1mL
Introduction: Messenger ribonucleic acid (mRNA) initiates protein translation in the cytoplasm without entering the nucleus, enabling faster protein expression than DNA transfection and avoiding the risk of gene integration. EZ Trans mRNA transfection reagent is a transfection reagent that combines the advantages of cationic polymers and liposomes, synergistically promoting the entry of transfection complexes into cells and their subsequent release in the cytoplasm. It can achieve efficient and low toxicity transfection effects in various cell types.
MRNA transfection efficiency display chart
On the day before transfection, 293T, Huh-7, BV2, and B16F10 cells were seeded into 24 well plates to ensure cell density of 60-80% or higher during transfection. Perform green fluorescent protein GFP mRNA transfection according to the operating instructions of the mRNA transfection reagent. After 24 hours of transfection, the transfection efficiency of each cell line was evaluated using fluorescence microscopy. The results showed that the transfected cells were in good condition and the fluorescence efficiency exceeded 90%.
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