Efficient removal of cell black fungus remover ensures the quality of cell culture
During the process of cell culture, contamination by black gum mites is a common and challenging issue. The competitive growth between black glue worms and cells not only leads to slow cell growth and poor state, but in severe cases, it can even cause cell death, which has a great impact on cell culture and subsequent experiments. In order to effectively address this issue, cell melanocyte removers have emerged. This article introduces the working principle, usage method, and precautions of cell black fungus remover.
1、 The working principle of cell melanocyte remover
The main component of cell black fungus remover is antibiotics, which have efficient and specific clearing effects. It can accurately identify and kill black glue worms in serum, culture medium, and cells, while also having a certain clearing effect on common bacteria. While removing black gum mites, these removers have little effect on the growth and proliferation of cells themselves, thus protecting precious cells to a large extent.
2、 The usage method of cell black fungus remover
Judging pollution: Firstly, it is necessary to determine whether the cells have been contaminated by black glue worms. Usually, when observing cells under a microscope, if many small black spots are found around the cells and in the culture medium, and the number of small black spots gradually increases with the extension of culture time, and changing the culture medium or washing the cells is ineffective, it can be preliminarily judged as contamination by black fungus.
Prepare the remover: According to the instructions of the selected cell black fungus remover, dilute it to the appropriate concentration according to the recommended ratio. Usually, the recommended dilution ratio is 1:500 or higher, depending on the brand of remover and cell type used.
Handling cells: Discard the old culture medium and wash the cells 2-3 times with PBS to remove residual black glue worms and bacteria. Then, add a new complete culture medium containing a black fungus remover and continue to culture the cells.
Continuous treatment: Change the medium or passage once every 2 days using a complete culture medium containing a black fungus remover, and treat continuously for about 3 times. If the pollution is severe, the treatment time can be extended to 3-5 days or longer until the black gum mites are completely removed.
3、 Precautions
Aseptic operation: When using cell black fungus remover, strict adherence to aseptic operation procedures should be followed to prevent new contamination from occurring.
Cell state monitoring: During the processing, the state and growth of cells should be closely monitored to ensure that the impact of the removal agent on the cells is within an acceptable range.
Preventive measures: In order to prevent cells from being contaminated by black glue worms again, it is recommended to continue using removers for prevention after the treatment is completed. Meanwhile, strengthening environmental control and hygiene management in laboratories is also an important measure to prevent contamination by black glue worms.
In summary, cell black fungus remover is an effective tool for dealing with black fungus contamination in cell culture. By using these removers correctly, we can effectively ensure the quality of cell culture and provide strong support for scientific research.
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