Cell cryopreservation solution, as a liquid environment for freezing cells, provides nutrients and protection to the cells. It can lower the freezing point, increase the permeability of the cell membrane to water, and allow water to seep out of the cells before freezing, preventing or reducing the damage of ice crystals to the cells. It temporarily removes the cells from their growth state and preserves their cellular characteristics. When needed, it can be directly revived to restore cell activity. So, which one is better, cs10 cryopreservation solution or Li Ji cell cryopreservation solution? How to choose? Let me introduce it to you below.
一、 Introduction to CS10 Cryopreservation Liquid and Li Ji Cell Cryopreservation Liquid Products
1. CryoStor CS10: With a unique formula, it is serum-free, animal free, and contains a 10% dimethyl sulfoxide (DMSO) cryopreservation solution. Designed to protect cells at low temperatures (-80 ° C -196 ° C), CryoStor CS10 provides a safe and protective environment for cells and tissues during freezing, storage, and thawing processes. CryoStor CS10 is recommended for cryopreservation of stem cells, tissue samples, human peripheral blood, CHO cells, myeloma cells, hybridoma cells, bone marrow mesenchymal stem cells, human embryos and induced pluripotent stem cells (ES cells and iPS cells), as well as other highly sensitive cell types. CryoStor CS10 is produced under USP grade cGMP conditions.
2. Heyuan Liji Rapid Cell Cryopreservation Liquid (Serum free, Protein free): Suitable for the vast majority of tumor cells and conventional cells, most stem cells (please follow the official website for the types of cells tested by Heyuan Liji), and frozen cells can be stored at -80 ℃ for a long time. Free from animal derived proteins and serum components, it can effectively reduce contamination from various bacteria, viruses, and mycoplasma, ensuring the safety of frozen cells. This cryopreservation solution contains various cellular nutrients such as DMSO and glucose, which improve cell survival rate and vitality. It is also suitable for serum-free culture of cells and protein expressing cells.
二、Advantages of CS10 Cryopreservation Liquid and Li Ji Cell Cryopreservation Liquid Products
brand | advantage |
cs10冻存液 | 1. Open and use immediately; 2. No serum and protein, no animal derived ingredients, no endotoxins; 3. USP grade cGMP production; 4. High cell survival and recovery rates. |
Li Ji Cell Cryopreservation Liquid |
1. High survival rate and strong activity: After cell recovery, the survival rate is high, the cell activity is strong, and it does not affect cell function. 2. Easy to operate: No need for program cooling, can be directly frozen in a -80 ℃ refrigerator. 3. High safety: The ingredients are clear and do not contain animal derived ingredients, ensuring safety. 4. Ready to use and take: No additional preparation required, convenient and fast. |
三、Precautions for using frozen liquid products
1. After adding frozen storage solution and packaging, cells should be stored outside for a shorter period of time and transferred to a -80 ℃ ultra-low temperature freezer as soon as possible.
2. For cryopreservation of stem cells (ES cells), it is recommended to conduct experimental cell cryopreservation for at least one week before use
After confirming the performance, proceed with formal cryopreservation.
3. This product contains 10% DMSO, and some cells sensitive to DMSO are recommended to undergo at least one week of experimental cell freezing with this product
Incubate and confirm performance before formal freezing.
When cells are revived, the frozen cells taken out should be immediately placed in a 37 ℃ water bath and gently shaken. Rapid thawing can help improve recovery efficiency.
5. For fragile or precious cells, it is recommended to store them overnight at -80 ° C and then transfer them to liquid nitrogen tanks for long-term storage.
The above is the complete introduction of "Which is better, cs10 cryovial or Li Ji cell cryovial? How to choose?" The selection can be made according to the needs of scientific research.
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