Cell lysis refers to the process of breaking down the structure of a cell to release its internal components. The purpose is to obtain intracellular biomolecules such as proteins, nucleic acids, etc. for further analysis and research. And the lysate is widely used in biomedical, molecular biology, pharmacy, microbiology, biochemical analysis and other fields.
Types and product recommendations of cell lysate
1. NP40 lysis buffer
✔ The main components are 50mM Tris (pH 7.4), 150mM NaCl, and 1% NP-40. NP-40, as a mild non-ionic detergent, can destroy cell membranes at a concentration of 1%, with weak damage to nuclear membranes. It can bind to buffer solutions to obtain cytoplasmic proteins.
✔ Suitable for non denaturing dissolution of membrane proteins. Commonly used in experiments such as Western, IP, co IP, and ELISA. If it is necessary to lyse the cell nuclear membrane to obtain nuclear proteins, the methods of 1% NP40+0.1% SDS (WB experiment) or 1% NP40+ultrasound (IP experiment) can be used.
2. SDS lysis buffer
✔ The main components are 50mM Tris (pH 8.1) and 1% SDS, which is a powerful cell tissue lysis buffer that can lyse the nuclear membrane
✔ Commonly used in routine Western, ChIP and other experiments, it can effectively destroy cell structure and release intracellular components, but has a significant impact on protein structure.
3. Red blood cell lysate
✔ The main components are NH ₄ Cl, KHCO ∝, and Na ₂ EDTA. Ammonium chloride is the main effector substance, which causes a difference in ion concentration inside and outside the cell, causing red blood cells to swell and lyse, while having a relatively small impact on white blood cells. This lysate is a mild method for removing red blood cells.
✔ Commonly used for the removal of red blood cells in experiments such as tissue cell protein and nucleic acid extraction, lymphocyte separation and purification, etc. The lysed tissue cells can be further used for various experimental studies.
4. IP cracking solution
✔ The components are 25mM Tris HCl (pH 7.4), 150mM NaCl, 1% NP-40, 1% mM EDTA, and 5% glycerol. It is an improved RIPA lysis buffer with moderate strength efficacy, which can efficiently dissolve cellular proteins without releasing chromosomal DNA or damaging protein complexes like RIPA.
✔ Suitable for downstream immunoprecipitation or affinity adsorption applications.
5. RIPA cracking solution
✔ The lysis buffer contains 25mM Tris HCl (pH 7.6), 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, and 0.1% sodium dodecyl sulfate (SDS), which is a traditional rapid lysis buffer.
✔ Protein samples can be obtained for routine WB, IP and other experiments, with strong lysis ability and effective dissolution of cellular proteins.
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