During the process of cell culture, contamination by black gum mites is a common and challenging issue. These tiny organisms not only affect the normal growth of cells, but may also lead to deviations in experimental results. In order to effectively address this issue, a black gum worm removal kit has been developed, becoming a clean guard in cell culture.
1、 The hazards of black glue insect pollution
Black gum worm, also known as "gum worm" or "coke worm", is a microorganism that often coexists with cells. As cells are passaged, antibiotics are often ineffective against them. Cells contaminated with black glue worms can exhibit various abnormal behaviors, such as irregular Brownian motion with many small black spots in the intercellular space, slow cell growth, deterioration of state, and changes in cell morphology. These abnormalities not only affect the normal physiological functions of cells, but may also interfere with the accuracy of experimental results.
2、 The principle and composition of the black glue insect removal kit
The black gum worm removal kit is a product specifically designed to remove black gum worms from cells, serum, and culture media. The principle is usually to inhibit or kill black gum mites through specific chemical components or biological agents, while ensuring minimal toxicity to the cells themselves. The ingredients of different brands of test kits may vary, but most of them contain antibiotics or other active ingredients that can effectively remove black glue worms.
3、 Steps for using the black glue insect removal kit
When using the black glue insect removal kit, the following steps are usually required:
Preparation of reagents: Based on the characteristics of the cultured cells and the instructions of the kit, prepare the reagents in the kit into the corresponding complete culture medium.
Handling cells: Discard the old culture medium, wash the cells thoroughly with buffer solutions such as PBS, and then add fresh culture medium containing a black fungus removal reagent.
Cultivation observation: Place the processed cells in the incubator for further cultivation, and observe the cell status and clearance of black glue worms. According to the instructions and experimental requirements of the reagent kit, continuous cultivation for a period of time (such as 1-2 weeks) may be required to achieve clearance effect.
Follow up treatment: If the pollution is severe, the drug concentration can be increased or the treatment time can be extended as appropriate. After the processing is completed, the cell status should be checked again to ensure that the black fungus has been effectively removed.
4、 Precautions
When using the black glue insect removal kit, the following points should also be noted:
Strictly follow the instructions of the reagent kit to avoid misuse or excessive use.
During the processing, it is necessary to wear experimental clothing and disposable gloves and other protective equipment to ensure the safety of the experiment.
The processed cells should be thoroughly observed and tested to ensure that the black glue worms have been completely eliminated and the cell state is good.
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