During the process of cell culture, contamination by black gum mites is a common and challenging issue. These tiny pollutants appear as small black dots under the microscope, sometimes in the form of dots, sometimes in small flakes, and their motion is in situ vibration (similar to Brownian motion), which has a serious impact on cell growth and experimental results. In order to effectively remove these pollutants, scientists have developed a black gum worm removal kit, which provides strong support for cell culture.
1、 The basic components of the black glue insect removal kit
The black gum worm removal kit usually contains one or more highly effective cleaning reagents for black gum worms. The main components of these reagents are antibiotics or other compounds with bactericidal effects, which can specifically act on black glue worms without affecting the normal growth of cells. In addition, the reagent kit may also contain some auxiliary reagents, such as PBS buffer, complete culture medium, etc., for cell cleaning and reagent dilution.
2、 Instructions for using the black glue insect removal kit
Preparation work: Firstly, it is necessary to ensure a clean cell culture environment and avoid cross contamination. At the same time, prepare the necessary reagents and equipment, such as pipettes, centrifuge tubes, microscopes, etc.
Reagent dilution: Dilute the clearing reagent to the appropriate concentration according to the instructions of the reagent kit. The dilution ratio varies depending on the reagent brand and cell type, so it is necessary to carefully read the instructions and strictly follow the instructions.
Cell cleaning: Discard the old culture medium and clean the cells with PBS buffer to remove residual serum and impurities in the culture medium.
Reagent addition: Add the diluted clearing reagent to a new culture medium, and then add it to the cell culture dish. Ensure uniform distribution of reagents and avoid mechanical damage to cells.
Cultivation observation: Place the processed cells in a culture box for cultivation, and regularly observe the cell status and clearance effect. According to the instructions, it may be necessary to handle multiple cycles continuously.
3、 Precautions for the Black Gum Insect Removal Kit
Operating standards: During use, sterile operating standards should be strictly followed to avoid introducing new sources of contamination.
Reagent storage: The removal reagent for black glue worms usually needs to be stored under specific temperature conditions (such as -20 ℃) and avoid repeated freezing and thawing to ensure its activity.
Cytotoxicity: Although the black glue insect removal reagent is non-toxic or low toxic to the cells themselves, attention should still be paid to its impact on cell growth during use. Therefore, cytotoxicity testing should be conducted before processing.
Processing cycle: The processing cycle may vary depending on the degree of contamination and cell type. Therefore, adjustments need to be made according to the actual situation during use.
Back
