"Heyuan Liji" is a joint venture between Heyuan Biotechnology (stock code: 688238) and Liji Biotechnology, specializing in the "Liji Biotechnology" and "Life-ilab" reagent brands

Language：

中文 English

He Yuan Li Ji

021-50778506

Hot Search：

Product Center

Publications

Technical Services

Information

Resource Centre

About Li Ji

Back

Cell Biology

Western Blot

Electrophoresis

Fluorescent labeling dye

PCR&q-PCR

Synthesis and extraction of nucleic acids

Microbial culture

Elisa

Biochemical reagents

Instrument consumables

Back

Information

Paper Prize

Back

Services

Vector for gene therapy

Genetic manipulation

Cell experiments

Animal testing

Other special services

Back

Back

Back

Why is the repeatability of CCK8 detection data poor?

Pubtime：2024-05-17

View volume：177

1. The reagents in the outermost circle of the well plate are prone to volatilization. It is recommended to only add culture medium to the outermost circle of the well and not use it as a measuring well. 2. CCK-8 sticks to the wall. It is recommended to add CCK-8 and gently tap the culture plate to help mix. 3. If there are too many or too few cells, it is recommended to explore the conditions within the range of 1000-100000 cells per well in advance. 4. Bubbles inside the well interfere with the enzyme-linked immunosorbent assay (ELISA) detection. Before detection, it is necessary to ensure that there are no bubbles in each well.

Previous：Does the drug affect the determination of CCK-8 during cell drug addition experiments?

Next：Why is the absorbance value measured by the CCK8 kit low?

Group Website