Does the drug affect the determination of CCK-8 during cell drug addition experiments?-FAQ-He Yuan Li Ji

Does the drug affect the determination of CCK-8 during cell drug addition experiments?

Pubtime：2024-05-17

View volume：177

1. Note that if the drug has reducing properties (such as vitamin C), it will undergo a color reaction with CCK-8, increasing the absorbance. 2. The metals in the drug have an impact on the color development of CCK-8: when the final concentration is 1mM, lead chloride, iron oxide, and copper sulfate will inhibit 5%, 15%, and 90% of the color development reaction, resulting in a decrease in sensitivity. If the final concentration is 10mM, it will be 100% inhibited. Solution: Firstly, it is necessary to confirm whether the drug has been absorbed. Add CCK-8 to the culture medium containing the drug and measure the absorbance at 450nm. If its absorbance is higher than that of the culture medium without the drug (with CCK-8 added), it indicates that the drug has an effect. The culture medium can be changed before adding CCK-8 to remove the effect of the drug. 3. Due to the possibility of oxidation-reduction reaction substances in the culture medium, it is recommended to use a well for testing before the formal experiment. It is necessary to confirm whether the culture medium and CCK-8 react first. Generally, the normal OD value should be below 0.4.

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