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What is the difference between Annexin V and TUNEL?

Pubtime：2024-06-04

View volume：131

Terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL) is a staining method used to identify DNA fragmentation sites within cells - a hallmark feature of late stage cell apoptosis. It uses enzyme terminal deoxynucleotide transferase (TdT) to connect modified dNTPs (such as dUTP) to the 3 '- hydroxyl end of fragmented DNA strands. DNTPs are typically modified with fluorescent groups to facilitate quantification and/or visualization.

Annexin V staining identifies the early stages of cell apoptosis by binding to PS residues exposed outside the cell due to loss of membrane asymmetry. Annexin V is commonly labeled with fluorescent groups such as FITC to promote the detection of apoptotic cells.

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