"Heyuan Liji" is a joint venture between Heyuan Biotechnology (stock code: 688238) and Liji Biotechnology, specializing in the "Liji Biotechnology" and "Life-ilab" reagent brands

Language: English

What is Annexin V/PI assay?

Pubtime:2024-06-04
View volume:24

The Annexin V/PI assay is used to distinguish early apoptotic cells through Annexin V labeling and necrotic/dead cells through propiconazole (PI) labeling. In the early stage of apoptosis, the cell membrane begins to lose asymmetry, leading to the translocation of phosphatidylserine (PS) to the outer surface of the membrane, where it can be detected by annexin V conjugates. However, in the case of membrane rupture, such as necrosis, annexin V conjugates can enter and bind to PS residues in the inner membrane, leading to false positive results. Therefore, it is recommended to use non membrane penetrating nuclear dyes (such as PI) in combination with annexin V conjugates. This allows users to distinguish between early apoptotic cells (Annexin V staining), late apoptotic cells (Annexin V and PI staining), and necrotic cells (PI staining only).

联系方式

座机电话:021-50778506 
业务经理:18616108315

地址:上海市浦东新区国际医学园区紫萍路908弄27号楼4层

邮箱:lsj0027@obiosh.com

Copyright (C) 2015-2016 Heyuan Liji (Shanghai) Biotechnology Co., Ltd. All Rights Reserved

沪ICP备16001244号-1

电话咨询

18616108315

微信咨询

在线客服

申请使用

Back to the top