When is the best time for cell cryopreservation and resuscitation?-Frequently asked questions-He Yuan Li Ji

When is the best time for cell cryopreservation and resuscitation?

Pubtime：2024-06-04

View volume：150

There are three stages of cell growth in vitro: latency, exponential growth and plateau. The incubation period is usually just when the cells begin to attach to the matrix and stretch the cytoskeleton. The metabolic activity focuses on the expression of adhesion and cytoskeleton proteins, and the cell quantity hardly increases during this period. After that, the cells began to grow exponentially, the transcription and translation of nucleic acids were vigorous, the DNA was unrotated and paired frequently, the material in the cells transferred rapidly, and the number of cells increased rapidly. If cryopreservation at this time, in effect, forces the cell to freeze at some point in its metabolism, it is bound to cause mechanical damage to unrotated DNA, RNA and proteins in transcription and translation, increase the risk of errors in individual links. With the increase of the number of cells, the confluence of cells reached more than 90% in the culture container. Most of the cells stopped high metabolism and proliferation because of“Contact inhibition”, and the intracellular activity tended to be smooth. Therefore, we propose to freeze the cells just into the plateau period, both to obtain sufficient cells, but not to cause excessive mechanical damage to the cells.

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