What should be noted for efficient cell cryopreservation?-Frequently asked questions-He Yuan Li Ji

What should be noted for efficient cell cryopreservation?

Pubtime：2024-06-04

View volume：117

1) slow freezing and freezing can dehydrate the cells gradually, and the cells will not be damaged by the formation of large ice crystals. On the contrary, the formation of large crystals in cells may cause damage and rupture of cell membranes and organelles. 2) the viability and concentration of cells should be cryopreserved when the density is about 80-90% and the viability is more than 90% . The survival rate of cells with poor viability after cryopreservation is very low. Therefore, it is necessary to cryopreservation during the period of vigorous cell division. 3) cryopreservation density when the cell density is less than 5x105 cells/mL, it is difficult to revive successfully. 4) frozen storage tube cover frozen storage tube cover must be tightened, otherwise the water bath recovery will cause water infiltration pollution. 5) the cells could not be stored in -80 ° C refrigerator for a long time.

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