"Heyuan Liji" is a joint venture between Heyuan Biotechnology (stock code: 688238) and Liji Biotechnology, specializing in the "Liji Biotechnology" and "Life-ilab" reagent brands
Can Trizol be added after centrifugation of adherent cells after digestion? Does it have an impact?
Sure, resuspend with a small amount of PBs and extract with triazol, but the extraction should be fast and cannot be stored because the cells are living and their expression is affected by the external environment. If they have been stored for two hours, it is recommended to redo.