How to deal with pancreatic tissue RNA always sticking to the mortar during grinding? Is there any good way?-Frequently asked questions-He Yuan Li Ji

How to deal with pancreatic tissue RNA always sticking to the mortar during grinding? Is there any good way?

Pubtime：2024-07-02

View volume：150

Immediately immerse the pancreatic tissue in liquid nitrogen after removal, then transfer it to a mortar (the mortar needs to be dried at 180 degrees for 4-8 hours to remove RNAase), continue grinding, add liquid nitrogen while grinding, and grind it into powder. At this point, it is important to pay attention to the replenishment of liquid nitrogen. If the tissue breaks down, there may be a situation where the tissue sticks to the mortar. Grind into powder and addTrizol，At this point, since Trizol will also freeze at low temperatures, continue grinding until Trizol dissolves and becomes clear. Then centrifuge and proceed with the following steps. You can also use a homogenizer, which is very cheap and simple. Of course, it needs to be processed with RNAase. Grind the tissue into a homogenate on ice.

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