"Heyuan Liji" is a joint venture between Heyuan Biotechnology (stock code: 688238) and Liji Biotechnology, specializing in the "Liji Biotechnology" and "Life-ilab" reagent brands

Language：

中文 English

He Yuan Li Ji

021-50778506

Hot Search：

Product Center

Publications

Technical Services

Information

Resource Centre

About Li Ji

Back

Cell Biology

Western Blot

Electrophoresis

Fluorescent labeling dye

PCR&q-PCR

Synthesis and extraction of nucleic acids

Microbial culture

Elisa

Biochemical reagents

Instrument consumables

Back

Information

Paper Prize

Back

Services

Vector for gene therapy

Genetic manipulation

Cell experiments

Animal testing

Other special services

Back

Back

Back

How to determine bacterial contamination in cultured cells

Pubtime：2024-08-02

View volume：143

After overnight cultivation of cells contaminated with bacteria, the culture medium will significantly turn yellow, and bacterial sediment can be seen with the naked eye in the culture bottle. Shaking the culture medium will cause turbidity, and under a microscope, cells can be observed with fine sand covering the entire bottom of the culture bottle. The distinction between cell debris and contamination can be determined by overnight cultivation. The main components that do not become cloudy overnight are cell debris and metabolites, while those that become significantly yellow and cloudy overnight can be identified as contamination. Bacterial contamination of cells usually erupts to a visible state within 1-2 days, and bacterial contamination generally does not cross contaminate. Timely disposal of contaminated cells is sufficient.

Previous：What are the precautions for using pre stained protein markers

Next：What is the difference between organizational transport fluid and RNA later?

Group Website