"Heyuan Liji" is a joint venture between Heyuan Biotechnology (stock code: 688238) and Liji Biotechnology, specializing in the "Liji Biotechnology" and "Life-ilab" reagent brands

Language：

中文 English

He Yuan Li Ji

021-50778506

Hot Search：

Product Center

Publications

Technical Services

Information

Resource Centre

About Li Ji

Back

Cell Biology

Western Blot

Electrophoresis

Fluorescent labeling dye

PCR&q-PCR

Synthesis and extraction of nucleic acids

Microbial culture

Elisa

Biochemical reagents

Instrument consumables

Back

Information

Paper Prize

Back

Services

Vector for gene therapy

Genetic manipulation

Cell experiments

Animal testing

Other special services

Back

Back

Frequently asked questions

Back

About us

Agent

Recruiting wise men

What are the precautions for using pre stained protein markers

Pubtime：2024-08-02

View volume：120

1) Due to the coupling effect between pre dyed protein markers and dyes, the migration rate of markers varies in different buffer solutions. Therefore, it is important to choose an appropriate buffer system before use. If the electrophoresis buffer is not mentioned in the user manual, it is recommended to use a non pre stained protein marker to calibrate the molecular weight of the pre stained protein marker first;

2) In low concentration gels, low molecular weight proteins will swim at the front edge of the dye;

3) When performing Western blot on high molecular weight proteins, it is necessary to extend the transfer time or increase the transfer voltage.

Previous：Can pre stained protein markers be stained with Coomassie Brilliant Blue?

Next：How to determine bacterial contamination in cultured cells

Group Website