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What are the precautions for using pre stained protein markers

Pubtime：2024-08-02

View volume：161

1) Due to the coupling effect between pre dyed protein markers and dyes, the migration rate of markers varies in different buffer solutions. Therefore, it is important to choose an appropriate buffer system before use. If the electrophoresis buffer is not mentioned in the user manual, it is recommended to use a non pre stained protein marker to calibrate the molecular weight of the pre stained protein marker first;

2) In low concentration gels, low molecular weight proteins will swim at the front edge of the dye;

3) When performing Western blot on high molecular weight proteins, it is necessary to extend the transfer time or increase the transfer voltage.

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