"Heyuan Liji" is a joint venture between Heyuan Biotechnology (stock code: 688238) and Liji Biotechnology, specializing in the "Liji Biotechnology" and "Life-ilab" reagent brands
How to perform gradient replacement of serum?
The replacement method is as follows: during the complete culture medium preparation process, mix 25% new serum with 75% original serum and culture for 1-2 passages; Then mix 50% new serum and 50% original serum for subculture; Then mix 75% new serum and 25% original serum for subculture; Finally, the cells were cultured and passaged entirely using new serum.