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How to perform gradient replacement of serum?

Pubtime:2024-04-02
View volume:228

The replacement method is as follows: during the complete culture medium preparation process, mix 25% new serum with 75% original serum and culture for 1-2 passages; Then mix 50% new serum and 50% original serum for subculture; Then mix 75% new serum and 25% original serum for subculture; Finally, the cells were cultured and passaged entirely using new serum.

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