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Should DMSO be removed immediately when thawing and culturing cells in cryovials?
Except for a few cells specifically marked as sensitive to DMSO, the vast majority of cell lines (including suspended cells) can be directly placed in 10-15ml of fresh culture medium after thawing, and the fresh culture medium can be replaced the next day to remove DMSO. This can avoid the problem of most thawed cells being unable to grow or adhere.