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How should suspended cells be passaged?
Generally, it is only necessary to continuously add fresh culture medium to the original culture bottle and dilute the cell concentration. If there is too much culture medium, the mouth of the culture bottle can be slightly raised until it cannot accommodate it. When dividing the bottles, take out a portion of the culture medium containing cells and transfer it to another new culture bottle. Add fresh culture medium and dilute to the appropriate concentration. Repeat the above steps.