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Should DMSO be removed immediately when thawing and culturing cells in cryovials?
Except for a few cells specifically marked as sensitive to DMSO, the vast majority of cell lines (including suspended cells) should be placed directly into a culture flask containing 10-15 ml of fresh culture medium after thawing. The fresh culture medium should be replaced the next day to remove DMSO, which can avoid the problem of most thawed cells being unable to grow or adhere.