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What is the concentration of trypsin EDTA used during the subculture of adherent cells? How should it be handled?
The commonly used concentration of trypsin EDTA is 0.05% trypsin 0.53 mM EDTA Na4. After the first bottle opening, a small amount should be immediately divided into sterile test tubes and stored at -20 ℃ to avoid repeated freezing and thawing, which can reduce the activity of trypsin and reduce the chance of contamination.