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What are the grades of DMSO and the method of sterile filtration?

Pubtime:2024-04-16
View volume:222

The DMSO grade used for cryopreservation must be tissue culture grade DMSO (such as Sigma D2650), which is already in a sterile state. After the first bottle opening, a small amount should be immediately divided into sterile test tubes and stored at 4 ℃ to avoid repeated freezing and thawing that may cause DMSO cracking and release harmful substances, and to reduce the chance of contamination. To filter DMSO, a DMSO resistant Nylon material filter membrane must be used.

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