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What are the methods for cryopreservation of cells?

Pubtime：2024-04-16

View volume：222

Freezing preservation method 1: Place the freezing tube at 4 ℃ for 30-60 minutes → (-20 ℃ for 30 minutes *) → -80 ℃ for 16-18 hours (or overnight) → store in a liquid nitrogen tank vapor phase for long-term storage. Cryopreservation method 2: Place the freezing tube in a programmable cooling machine that has been programmed to cool down 1-3 ℃ per minute to below -80 ℃, and then store it in a liquid nitrogen tank vapor phase for long-term storage*- At 20 ℃, it should not exceed 1 hour to prevent excessive ice crystals from causing massive cell death. This step can also be skipped and placed in a -80 ℃ freezer, but the survival rate is slightly reduced.

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