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Why do we need to conduct cell passaging culture?

Pubtime：2024-04-23

View volume：191

When the proliferation of cultured cells reaches a certain density, density inhibition will occur, manifested as a gradual slowdown or even cessation of cell growth and division speed. Adherent cells will grow into a dense monolayer in the culture bottle (suspended cells will fill the entire volume of culture medium), covering the bottom of the culture bottle. If not packaged and cultured in a timely manner, i.e., subculturing, the cells will gradually deteriorate and die. Transferring the cultured cells from one container to another at an appropriate ratio to expand the culture is called subculturing.

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